White and red blood cells

The Immunotherapy and Hematopoietic Knowledge Center is dedicated to sharing interesting articles and resources related to hematopoietic research. Click on the links below to explore different articles. 

If there is a topic you would like to see highlighted, please send an email to hematopoietics at lonza.com.

Image of hematopoietic cell types starting with hematopoietic stem cell down to fully committed lymphocytes, macrophages, dendritic cells, etc.

Boosting the Immune system – Steps to Take for Successful Substrate Delivery


Optimizing the immune system using novel genome editing techniques provides new tools to treat a broad range of diseases such as leukemia, solid-tumors or HIV.


For this purpose, substrate delivery into hematopoietic and blood cells is essential. This article covers the topics transfection efficiency and cell viability combined with preservation of functionality for various immune cell types, focusing on the Nucleofector™ Technology as transfection method. Read more...

Differentiation of monocytes - thumbnail

Tuning Macrophages for Immunotherapy


During initial differentiation from monocytes, macrophages can differentiate into two types of macrophages: M1, considered kill-type macrophages, or M2, considered repair-type macrophages.


The ratio of M1 to M2-type macrophages appears to be relevant in the development of cancer. The latest immunotherapy research proposes two options to increase the ratio of M1 macrophages. Read more...

Inflammatory bowel disease graphic

Rising Global Need for Treatment of Inflammatory Bowel Disease: Cytokine Blocking Immunotherapies Show Potential


Inflammatory Bowel Disease (IBD) is an autoimmune disorder that causes chronic inflammation throughout all or part of a person’s gastrointestinal tract.


The role of immune cells in the inflammation pathway has been a new target for IBD therapies in recent years. Cytokine blocking compounds have been developed and are already showing success in blocking the immune response. Read more...


Visual of B and T cells suppression of allergic reactions

Allergen Immunotherapy to the Rescue


The number of people worldwide suffering from some form of allergy is extremely high. For some of these allergy sufferers, allergen specific immunotherapy (allergen-SIT) has provided more than temporary relief.


The immunological mechanism of allergen-SIT is essentially the induction of persistent specific allergen tolerance. The allergen-specific memory T and B cell responses are altered, leading to immune tolerance. Read more...

 hIL-17 secretion determination graph

Validation of TH17 Cell Differentiation from Peripheral Blood CD4+T Cells


The combination of differentiation procedure, cells, assessment methods and instrumentation provide a simplified, robust process for the creation and validation of activated immune cells. Read more...

Chimeric antigen receptors

A Molecular Spotlight on CAR-T Cells


Chimeric antigen receptor-redirected T cells (CAR-T cells) have become a promising tool in the treatment of hematopoietic malignancies as well as solid tumors.


CARs expressed on T cells target surface antigens in their native conformation, independent of the major histocompatibility complex. In CAR-T cell therapies the collection of patient’s T cells is followed by  Read more...

msc infographic thumbnail

Mesenchymal Stem Cells Infographic


Mesenchymal stem cells can differentiate into chondrocytes, adipocytes, and osteoclasts. They have extensive uses in both regenerative medicine and immunotherapy research Read more...

Image of NK cell subsets based on expression of CD56 and CD16

Natural Killer Cells Infographic


Natural Killer cells have an innate ability to recognize and destroy non-self cells. They are characterized as CD3- CD56+.  NK cell subsets are characterized by their expression of Read more...

schematic describing current genetic modification strategies to optimize nk cells for use in immunotherapy

Natural (Born) Killers - NK Cells in Immunotherapy 


Natural Killer (NK) cells are aptly named. They act like well-trained snipers, ready to target and destroy foreign cells at the drop of a hat.


They do not require prior sensitization, or exposure to foreign antigen, in order to kill. Instead, they have an innate, natural ability to recognize and destroy non-self cells.  


NK cells are broadly characterized as


PBMCs - The One Stop Immune Cell Shop


PBMCs are a mixed population of myeloid and lymphoid cells. The myeloid cells include monocytes, dendritic cells, and macrophages. Monocytes circulate through the blood to different tissues, where they differentiate into tissue-resident macrophages and dendritic cells.


Macrophages are the Pac-Man cells of the immune system that engulf and destroy foreign substances and cancerous cells. There are resident macrophages that maintain a steady-state of general tissue repair. In addition, there are also Read more...


Cord Blood-Derived HSCs - The Quest to Overcome Limited Cell Numbers 


Bone marrow and peripheral blood-derived HSCs allow for large numbers of CD34+ cells to be harvested and transplanted, but they are of limited utility if an HLA-matched donor is unavailable. Cord blood-derived hematopoietic stem cells offer a potential solution, as the cells tend to be more naïve, which may lead to fewer occurrences of host-versus-graft-disease (GVHD) in instances of HLA-mismatch.


Despite their potential in combating GVHD, cord blood-derived HSCs also pose a major challenge due to the limited number of cells available from Read more...

CD4+ helper T cell subsets image

T Cells - An Orchestrated Motley Crew 


The immune system is an intricate and fascinating network of many different cell types and signaling molecules that work together to protect the human body against foreign pathogens.


T cells are responsible for regulating the immune response, and much like a motley crew, they possess Read more...